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PYCARD 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00029108-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PYCARD full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 21.56
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PYCARD antibody (H00029108-B02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00029108-T02
    PYCARD transfected lysate.
    Western Blot
    QC Testing of H00029108-T02
    Lane 1: PYCARD transfected lysate ( 16.5 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • PYCARD
  • Gene Alias:
  • ASC,CARD5,MGC10332,TMS,TMS-1,TMS1
  • Gene Description:
  • PYD and CARD domain containing
  • Gene Summary:
  • This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
  • Other Designations:
  • apoptosis-associated speck-like protein containing a CARD,caspase recruitment domain protein 5,target of methylation-induced silencing-1
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