Products

Western Blot (Transfected lysate)

Western Blot analysis of NXT1 expression in transfected 293T cell line by NXT1 monoclonal antibody (M08), clone 4F11.

Lane 1: NXT1 transfected lysate(15.51 KDa).
Lane 2: Non-transfected lysate.

Western Blot (Recombinant protein)

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged NXT1 is approximately 0.3ng/ml as a capture antibody.

ELISA

RNAi Knockdown (Antibody validated)

Western blot analysis of NXT1 over-expressed 293 cell line, cotransfected with NXT1 Validated Chimera RNAi ( Cat # H00029107-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NXT1 monoclonal antibody (M08), clone 4F11 (Cat # H00029107-M08 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Immunofluorescence

Immunofluorescence of monoclonal antibody to NXT1 on HeLa cell . [antibody concentration 10 ug/ml]

Entrez GeneID

29107

GeneBank Accession#

BC000759

Protein Accession#

AAH00759

Gene Name

NXT1

Gene Alias

MTR2, P15

Gene Description

NTF2-like export factor 1

Omim ID

605811

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene is located in the nuclear envelope. It has protein similarity to nuclear transport factor 2. This protein functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1 (required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. The encoded protein heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq

Other Designations

NTX2-like export factor1|NUTF-like export factor 1|OTTHUMP00000030420