MRPL22 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MRPL22 transfected lysate
Western Blot
Lane 1: MRPL22 transfected lysate ( 22.77 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPL22 full-length
Host
Human
Theoretical MW (kDa)
22.77
Interspecies Antigen Sequence
Mouse (89); Rat (89)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPL22 antibody (H00029093-B01) by Western Blots.
SDS-PAGE Gel
MRPL22 transfected lysate
Western Blot
Lane 1: MRPL22 transfected lysate ( 22.77 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPL22
Entrez GeneID
29093GeneBank Accession#
BC012565Protein Accession#
AAH12565Gene Name
MRPL22
Gene Alias
DKFZp781F1071, HSPC158, L22mt, MRP-L22, MRP-L25, RPML25
Gene Description
mitochondrial ribosomal protein L22
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L22 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 4q. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
39S ribosomal protein L22, mitochondrial
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Interactome
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