UBE2T 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-UBE2T full-length
Host
Human
Theoretical MW (kDa)
22.5
Interspecies Antigen Sequence
Mouse (85); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-UBE2T antibody (H00029089-D01P) by Western Blots.
SDS-PAGE Gel
UBE2T transfected lysate.
Western Blot
Lane 1: UBE2T transfected lysate ( 22.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — UBE2T
Entrez GeneID
29089GeneBank Accession#
NM_014176.1Protein Accession#
NP_054895.1Gene Name
UBE2T
Gene Alias
HSPC150, PIG50
Gene Description
ubiquitin-conjugating enzyme E2T (putative)
Omim ID
610538Gene Ontology
HyperlinkGene Summary
The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM
Other Designations
HSPC150 protein similar to ubiquitin-conjugating enzyme|OTTHUMP00000038808|ubiquitin conjugating enzyme
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