CHMP4A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CHMP4A transfected lysate
Western Blot
Lane 1: CHMP4A transfected lysate ( 24.53 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CHMP4A full-length
Host
Human
Theoretical MW (kDa)
24.53
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CHMP4A antibody (H00029082-B01) by Western Blots.
SDS-PAGE Gel
CHMP4A transfected lysate
Western Blot
Lane 1: CHMP4A transfected lysate ( 24.53 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CHMP4A
Entrez GeneID
29082GeneBank Accession#
BC010893Protein Accession#
AAH10893Gene Name
CHMP4A
Gene Alias
C14orf123, CHMP4, CHMP4B, HSPC134, MGC142093, MGC142095, SNF7, SNF7-1, Shax2
Gene Description
chromatin modifying protein 4A
Omim ID
610051Gene Ontology
HyperlinkGene Summary
CHMP4A belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM
Other Designations
OTTHUMP00000164695|Snf7 homologue associated with Alix 2|charged multivesicular body protein 4A
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