MED4 monoclonal antibody (M01), clone 2B10
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of MED4 expression in transfected 293T cell line by MED4 monoclonal antibody (M01), clone 2B10.
Lane 1: MED4 transfected lysate(29.7 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MED4 is 1 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to MED4 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (55.44 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant MED4.
Immunogen
MED4 (AAH05189, 1 a.a. ~ 270 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAASSSGEKEKERLGGGLGVAGGNSTRERLLSALEDLEVLSRELIEMLAISRNQKLLQAGEENQVLELLIHRDGEFQELMKLALNQGKIHHEMQVLEKEVEKRDGDIQQLQKQLKEAEQILATAVYQAKEKLKSIEKARKGAISSEEIIKYAHRISASNAVCAPLTWVPGDPRRPYPTDLEMRSGLLGQMNNPSTNGVNGHLPGDALAAGRLPDVLAPQYPWQSNDMSMNMLPPNHSSDFLLEPPGHNKEDEDDVEIMSTDSSSSSSESD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (93)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (55.44 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MED4 expression in transfected 293T cell line by MED4 monoclonal antibody (M01), clone 2B10.
Lane 1: MED4 transfected lysate(29.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MED4 is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MED4 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MED4
Entrez GeneID
29079GeneBank Accession#
BC005189Protein Accession#
AAH05189Gene Name
MED4
Gene Alias
DRIP36, FLJ10956, HSPC126, RP11-90M2.2, TRAP36, VDRIP
Gene Description
mediator complex subunit 4
Omim ID
605718Gene Ontology
HyperlinkGene Summary
subunit 4 homolog|p36 TRAP/SMCC/PC2 subunit|vitamin D receptor interacting protein
Other Designations
OTTHUMP00000018390|mediator of RNA polymerase II transcription, subunit 4 homolog|p36 TRAP/SMCC/PC2 subunit|vitamin D receptor interacting protein
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Interactome
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Disease
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