Western blot analysis of FLVCR over-expressed 293 cell line, cotransfected with FLVCR Validated Chimera RNAi ( Cat # H00028982-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with FLVCR monoclonal antibody (M05), clone 4B2 (Cat # H00028982-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.