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MRPL42 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00028977-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MRPL42 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 15.73
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MRPL42 antibody (H00028977-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00028977-T01
    MRPL42 transfected lysate.
    Western Blot
    QC Testing of H00028977-T01
    Lane 1: MRPL42 transfected lysate ( 15.73 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • MRPL42
  • Gene Alias:
  • HSPC204,MRP-L31,MRPL31,MRPS32,PTD007,RPML31
  • Gene Description:
  • mitochondrial ribosomal protein L42
  • Gene Summary:
  • Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Further experiments will be needed to identify the specific subunit localization. Sequence analysis identified three transcript variants that encode two different isoforms. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provided by RefSeq
  • Other Designations:
  • mitochondrial ribosomal protein S32
  • Related Disease
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