MRPL42 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MRPL42 transfected lysate.
Western Blot
Lane 1: MRPL42 transfected lysate ( 15.73 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPL42 full-length
Host
Human
Theoretical MW (kDa)
15.73
Interspecies Antigen Sequence
Mouse (76); Rat (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPL42 antibody (H00028977-B01) by Western Blots.
SDS-PAGE Gel
MRPL42 transfected lysate.
Western Blot
Lane 1: MRPL42 transfected lysate ( 15.73 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPL42
Entrez GeneID
28977GeneBank Accession#
NM_014050.2Protein Accession#
NP_054769.1Gene Name
MRPL42
Gene Alias
HSPC204, MRP-L31, MRPL31, MRPS32, PTD007, RPML31
Gene Description
mitochondrial ribosomal protein L42
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a protein identified as belonging to both the 28S and the 39S subunits. Further experiments will be needed to identify the specific subunit localization. Sequence analysis identified three transcript variants that encode two different isoforms. Pseudogenes corresponding to this gene are found on chromosomes 4q, 6p, 6q, 7p, and 15q. [provided by RefSeq
Other Designations
mitochondrial ribosomal protein S32
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