MRPS28 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPS28 full-length
Host
Human
Theoretical MW (kDa)
20.8
Interspecies Antigen Sequence
Mouse (73)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPS28 antibody (H00028957-B01P) by Western Blots.
SDS-PAGE Gel
MRPS28 transfected lysate.
Western Blot
Lane 1: MRPS28 transfected lysate ( 20.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPS28
Entrez GeneID
28957GeneBank Accession#
NM_014018Protein Accession#
NP_054737.1Gene Name
MRPS28
Gene Alias
FLJ22853, HSPC007, MRP-S28, MRP-S35, MRPS35
Gene Description
mitochondrial ribosomal protein S28
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature. [provided by RefSeq
Other Designations
mitochondrial 28S ribosomal protein S35
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