PCLO 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
PCLO transfected lysate.
Western Blot
Lane 1: PCLO transfected lysate ( 39.27 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PCLO full-length
Host
Human
Theoretical MW (kDa)
39.27
Interspecies Antigen Sequence
Mouse (91); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PCLO antibody (H00027445-B01) by Western Blots.
SDS-PAGE Gel
PCLO transfected lysate.
Western Blot
Lane 1: PCLO transfected lysate ( 39.27 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PCLO
Entrez GeneID
27445GeneBank Accession#
XM_935987.1Protein Accession#
-Gene Name
PCLO
Gene Alias
ACZ, DKFZp779G1236
Gene Description
piccolo (presynaptic cytomatrix protein)
Omim ID
604918Gene Ontology
HyperlinkGene Summary
Synaptic vesicles dock and fuse in the active zone of the plasma membrane at chemical synapses. The presynaptic cytoskeletal matrix (PCM), which is associated with the active zone and is situated between synaptic vesicles, is thought to be involved in maintaining the neurotransmitter release site in register with the postsynaptic reception apparatus. The cycling of synaptic vesicles is a multistep process involving a number of proteins (see MIM 603215). Among the components of the PCM that orchestrate these events are Bassoon (BSN; MIM 604020), RIM (RBBP8; MIM 604124), Oboe, and Piccolo (PCLO).[supplied by OMIM
Other Designations
aczonin|piccolo
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Interactome
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Disease
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