RABGEF1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
RABGEF1 transfected lysate.
Western Blot
Lane 1: RABGEF1 transfected lysate ( 56.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RABGEF1 full-length
Host
Human
Theoretical MW (kDa)
56.9
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RABGEF1 antibody (H00027342-B01P) by Western Blots.
SDS-PAGE Gel
RABGEF1 transfected lysate.
Western Blot
Lane 1: RABGEF1 transfected lysate ( 56.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RABGEF1
Entrez GeneID
27342GeneBank Accession#
NM_014504Protein Accession#
NP_055319.1Gene Name
RABGEF1
Gene Alias
FLJ32302, RABEX5, rabex-5
Gene Description
RAB guanine nucleotide exchange factor (GEF) 1
Omim ID
609700Gene Ontology
HyperlinkGene Summary
RABGEF1 forms a complex with rabaptin-5 (RABPT5; MIM 603616) that is required for endocytic membrane fusion, and it serves as a specific guanine nucleotide exchange factor for RAB5 (RAB5A; MIM 179512) (Horiuchi et al., 1997 [PubMed 9323142]).[supplied by OMIM
Other Designations
Rab5 GDP/GTP exchange factor
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Interactome
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Disease
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