PRPF19 monoclonal antibody (M07), clone 2E5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PRPF19.
Immunogen
PRPF19 (NP_055317, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSLICSISNEVPEHPCVSPVSNHVYERRLIEKYIAENGTDPINNQPLSEEQLIDIKVAHPIRPKPPSATSIPAILKALQDEWDAVMLHSF
Host
Mouse
Reactivity
Human, Mouse, Rat
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.64 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PRPF19 monoclonal antibody (M07), clone 2E5. Western Blot analysis of PRPF19 expression in PC-12(Cat # L012V1 ).Western Blot (Cell lysate)
PRPF19 monoclonal antibody (M07), clone 2E5. Western Blot analysis of PRPF19 expression in NIH/3T3(Cat # L018V1 ).Western Blot (Cell lysate)
PRPF19 monoclonal antibody (M07), clone 2E5. Western Blot analysis of PRPF19 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of PRPF19 expression in transfected 293T cell line by PRPF19 monoclonal antibody (M07), clone 2E5.
Lane 1: PRPF19 transfected lysate(55.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PRPF19 is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PRPF19 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PRPF19
Entrez GeneID
27339GeneBank Accession#
NM_014502Protein Accession#
NP_055317Gene Name
PRPF19
Gene Alias
NMP200, PRP19, PSO4, SNEV, UBOX4, hPSO4
Gene Description
PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
Omim ID
608330Gene Ontology
HyperlinkGene Summary
PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).[supplied by OMIM
Other Designations
PRP19/PSO4 homolog|PRP19/PSO4 pre-mRNA processing factor 19 homolog|nuclear matrix protein NMP200 related to splicing factor PRP19
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Interactome
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Pathway
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