ANGPTL3 monoclonal antibody (M10), clone 3F11
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More Files
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Immunoprecipitation
Immunoprecipitation of ANGPTL3 transfected lysate using anti-ANGPTL3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ANGPTL3 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ANGPTL3 is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ANGPTL3.
Immunogen
ANGPTL3 (NP_055310.1, 361 a.a. ~ 460 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
HLVAITGNVPNAIPENKDLVFSTWDHKAKGHFNCPEGYSGGWWWHDECGENNLNGKYNKPRAKSKPERRRGLSWKSQNGRLYSIKSTKMLIHPTDSESFE
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (76); Rat (80)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of ANGPTL3 transfected lysate using anti-ANGPTL3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ANGPTL3 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ANGPTL3 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — ANGPTL3
Entrez GeneID
27329GeneBank Accession#
NM_014495Protein Accession#
NP_055310.1Gene Name
ANGPTL3
Gene Alias
ANGPT5
Gene Description
angiopoietin-like 3
Omim ID
604774Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is predominantly expressed in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain and the C-terminal fibrinogen (FBN)-like domain. The FBN-like domain in angiopoietin-like 3 protein was shown to bind alpha-5/beta-3 integrins, and this binding induced endothelial cell adhesion and migration. This protein may also play a role in the regulation of angiogenesis. [provided by RefSeq
Other Designations
OTTHUMP00000010719|angiopoietin 5
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Interactome
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Disease
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