PDLIM3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PDLIM3 transfected lysate
Western Blot
Lane 1: PDLIM3 transfected lysate ( 34.87 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PDLIM3 full-length
Host
Human
Theoretical MW (kDa)
34.87
Interspecies Antigen Sequence
Rat (68)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PDLIM3 antibody (H00027295-B01) by Western Blots.
SDS-PAGE Gel
PDLIM3 transfected lysate
Western Blot
Lane 1: PDLIM3 transfected lysate ( 34.87 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PDLIM3
Entrez GeneID
27295GeneBank Accession#
BC027870Protein Accession#
AAH27870Gene Name
PDLIM3
Gene Alias
ALP, DKFZp686L0362
Gene Description
PDZ and LIM domain 3
Omim ID
605889Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. This gene is found near a region of chromosome 4 that has been implicated in facioscapulohumeral muscular dystrophy, but this gene does not appear to be involved in the disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
PDZ and LIM domain protein 3|alpha-actinin-2-associated LIM protein|enigma homolog
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Interactome
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Disease
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