VPS4A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
VPS4A transfected lysate.
Western Blot
Lane 1: VPS4A transfected lysate ( 48.18 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-VPS4A full-length
Host
Human
Theoretical MW (kDa)
48.18
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-VPS4A antibody (H00027183-B01) by Western Blots.
SDS-PAGE Gel
VPS4A transfected lysate.
Western Blot
Lane 1: VPS4A transfected lysate ( 48.18 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — VPS4A
Entrez GeneID
27183GeneBank Accession#
NM_013245.2Protein Accession#
NP_037377.1Gene Name
VPS4A
Gene Alias
FLJ22197, SKD1, SKD2, VPS4, VPS4-1
Gene Description
vacuolar protein sorting 4 homolog A (S. cerevisiae)
Omim ID
609982Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16; the gene for the other resides on chromosome 18. [provided by RefSeq
Other Designations
SKD1-homolog|vacuolar protein sorting factor 4A|vacuolar sorting protein 4
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