SIGLEC8 (Human) Recombinant Protein (Q01)
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Specification
Product Description
Human SIGLEC8 partial ORF ( NP_055257, 18 a.a. - 121 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
EGDRQYGDGYLLQVQELVTVQEGLCVHVPCSFSYPQDGWTDSDPVHGYWFRAGDRPYQDAPVATNNPDREVQAETQGRFQLLGDIWSNDCSLSIRDARKRDKGS
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
37.18
Interspecies Antigen Sequence
Mouse (50); Rat (49)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — SIGLEC8
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Disease
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Publication Reference
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Intravenous immunoglobulin preparations contain anti-Siglec-8 autoantibodies.
von Gunten S, Vogel M, Schaub A, Stadler BM, Miescher S, Crocker PR, Simon HU.
The Journal of Allergy and Clinical Immunology 2007 Mar; 119(4):1005.
Application:Dot, anti–Siglec-8–depleted IVIg.
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Intravenous immunoglobulin preparations contain anti-Siglec-8 autoantibodies.
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