EIF2C2 monoclonal antibody (M01), clone 2E12-1C9(H00027161-M01)

EIF2C2 monoclonal antibody (M01), clone 2E12-1C9

Catalog # H00027161-M01

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Size:100 ug

Price: USD $ 335.00

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Images

Application

Western Blot (Cell lysate)

EIF2C2 monoclonal antibody (M01), clone 2E12-1C9. Western Blot analysis of EIF2C2 expression in MCF-7.

Protocol Download

Application

Western Blot (Transfected lysate)

Western Blot analysis of EIF2C2 expression in transfected 293T cell line by EIF2C2 monoclonal antibody (M01), clone 2E12-1C9.

Lane 1: EIF2C2 transfected lysate (Predicted MW: 42.4 KDa).
Lane 2: Non-transfected lysate.

Protocol Download

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Immunoperoxidase of monoclonal antibody to EIF2C2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]

Protocol Download

Application

Sandwich ELISA (Recombinant protein)

Detection limit for recombinant GST tagged EIF2C2 is 0.3 ng/ml as a capture antibody.

Protocol Download

Application

RNAi Knockdown (Antibody validated)

Western blot analysis of EIF2C2 over-expressed 293 cell line, cotransfected with EIF2C2 Validated Chimera RNAi ( Cat # H00027161-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2C2 monoclonal antibody (M01), clone 2E12-1C9 (Cat # H00027161-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Protocol Download

Application

Immunofluorescence

Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]

QC Test

Western Blot detection against Immunogen (68.03 KDa) .

Specification

Product Description

Mouse monoclonal antibody raised against a full length recombinant EIF2C2.

Immunogen

EIF2C2 (AAH07633.1, 483 a.a. ~ 859 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Sequence

MPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA

Host

Mouse

Reactivity

Human

Interspecies Antigen Sequence

Mouse (100); Rat (99)

Isotype

IgG1 Kappa

Quality Control Testing

Antibody Reactive Against Recombinant Protein.

Western Blot detection against Immunogen (68.03 KDa) .

Storage Buffer

In 1x PBS, pH 7.4

Storage Instruction

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Applications

Western Blot (Cell lysate)
EIF2C2 monoclonal antibody (M01), clone 2E12-1C9. Western Blot analysis of EIF2C2 expression in MCF-7.
Protocol Download

Western Blot (Transfected lysate)
Western Blot analysis of EIF2C2 expression in transfected 293T cell line by EIF2C2 monoclonal antibody (M01), clone 2E12-1C9.

Lane 1: EIF2C2 transfected lysate (Predicted MW: 42.4 KDa).
Lane 2: Non-transfected lysate.

Protocol Download

Western Blot (Recombinant protein)

Protocol Download

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to EIF2C2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
Protocol Download

Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged EIF2C2 is 0.3 ng/ml as a capture antibody.
Protocol Download

ELISA

RNAi Knockdown (Antibody validated)
Western blot analysis of EIF2C2 over-expressed 293 cell line, cotransfected with EIF2C2 Validated Chimera RNAi ( Cat # H00027161-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2C2 monoclonal antibody (M01), clone 2E12-1C9 (Cat # H00027161-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Protocol Download

Immunofluorescence
Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]
Gene Info — EIF2C2

Entrez GeneID
27161

GeneBank Accession#
BC007633.1

Protein Accession#
AAH07633.1

Gene Name

EIF2C2

Gene Alias

AGO2, MGC3183, Q10

Gene Description

eukaryotic translation initiation factor 2C, 2

Omim ID
606229

Gene Ontology
Hyperlink

Gene Summary

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Other Designations

argonaute 2
Interactome
- EIF2C2
Disease
Publication Reference
- Proliferation-promoting roles of linear and circular PVT1 are independent of their ability to bind miRNAs in B-cell lymphoma.
  Xing Zhao, Anke van den Berg, Melanie Winkle, Jasper Koerts, Annika Seitz, Debora de Jong, Bea Rutgers, Tineke van der Sluis, Emke Bakker, Joost Kluiver.
  
  International Journal of Biological Macromolecules 2023 Sep; 253(Pt 2):126744.
  
  Application：RIP-A, Human, CA46, DG75, KM-H2, L1236, L428, ST486, SUDHL4, SUDHL5, SUDHL10 cells.
- miR-204 suppresses porcine reproductive and respiratory syndrome virus (PRRSV) replication via inhibiting LC3B-mediated autophagy.
  Yao Yao, Sihan Li, Yingqi Zhu, Yangyang Xu, Siyuan Hao, Shuyuan Guo, Wen-Hai Feng.
  
  Virologica Sinica 2023 Jul; S1995-820X(23):00084.
  
  Application：IP, Pis, Porcine alveolar macrophages.
- Promiscuous splicing-derived hairpins are dominant substrates of tailing-mediated defense of miRNA biogenesis in mammals.
  Seungjae Lee, David Jee, Sid Srivastava, Acong Yang, Abhinav Ramidi, Renfu Shang, Diane Bortolamiol-Becet, Sébastien Pfeffer, Shuo Gu, Jiayu Wen, Eric C Lai.
  
  Cell Reports 2023 Feb; 42(2):112111.
  
  Application：IP, Human, K562 cells.
- Massively parallel identification of mRNA localization elements in primary cortical neurons.
  Samantha Mendonsa, Nicolai von Kügelgen, Sayaka Dantsuji, Maya Ron, Laura Breimann, Artem Baranovskii, Inga Lödige, Marieluise Kirchner, Meret Fischer, Nadja Zerna, Lucija Bujanic, Philipp Mertins, Igor Ulitsky, Marina Chekulaeva.
  
  Nature Neuroscience 2023 Mar; 26(3):394.
  
  Application：WB-Ti, Mouse, Mouse coritcal neurons.
- Identification of a Novel HBV Encoded miRNA Using Next Generation Sequencing.
  Vladimir Loukachov, Karel A van Dort, Louis Jansen, Henk W Reesink, Neeltje A Kootstra.
  
  Viruses 2022 Jun; 14(6):1223.
  
  Application：RNA Immunoprecipitation Chip (RIP) Assay, Human, HepG2 and HepG2.2.15 cells.
- A plant immune protein enables broad antitumor response by rescuing microRNA deficiency.
  Ye Qi, Li Ding, Siwen Zhang, Shengze Yao, Jennie Ong, Yi Li, Hong Wu, Peng Du.
  
  Cell 2022 May; 185(11):1888.
  
  Application：ChIP-Seq, Human, A-549 cells, HepG2 cells.
- Target-Dependent Coordinated Biogenesis of Secondary MicroRNAs by miR-146a Balances Macrophage Activation Processes.
  Susanta Chatterjee, Ishita Mukherjee, Shreya Bhattacharjee, Mainak Bose, Saikat Chakrabarti, Suvendra N Bhattacharyya.
  
  Molecular and Cellular Biology 2022 Apr; 42(4):e0045221.
  
  Application：WB, Mouse, RAW264.7 cells.
- The Role of the MYC/miR-150/MYB/ZDHHC11 Network in Hodgkin Lymphoma and Diffuse Large B-Cell Lymphoma.
  Lotteke J Y M Ziel-Swier, Yichen Liu, Annika Seitz, Debora de Jong, Jasper Koerts, Bea Rutgers, Rianne Veenstra, Fazlyn R Abdul Razak, Agnieszka Dzikiewicz-Krawczyk, Anke van den Berg, Joost Kluiver.
  
  Genes 2022 Jan; 13(2):227.
  
  Application：RIP-A, Human, L428, SUPHD1 cells.
- miR-424/503 modulates Wnt/β-catenin signaling in the mammary epithelium by targeting LRP6.
  Erin A Nekritz, Ruth Rodriguez-Barrueco, Koon-Kiu Yan, Meredith L Davis, Rachel L Werner, Laura Devis-Jauregui, Partha Mukhopadhyay, Jiyang Yu, David Llobet-Navas, Jose Silva.
  
  EMBO reports 2021 Oct; e53201.
  
  Application：IP, Human, MCF-10A cells.
- Distinct temporal expression of GW182 (TNRC6A) in neurons regulates dendritic arborization.
  Bharti Nawalpuri, Arpita Sharma, Sumantra Chattarji, Ravi S Muddashetty.
  
  Journal of Cell Science 2021 Aug; 134(16):jcs258465.
  
  Application：WB-Ce, WB-Ti, Mouse, Mouse brain, Mouse hippocampal neurons.
- Amyloid Beta oligomers prevents Lysosomal targeting of miRNP to stop its recycling and target repression in glial cells.
  Dipayan De, Suvendra N Bhattacharyya.
  
  Journal of Cell Science 2021 Jun; 134(11):jcs258360.
  
  Application：IF, WB-Ce, WB-Tr, Human, C6 glioblastoma cells, HEK 293 cells.
- TDP-43 aggregation induced by oxidative stress causes global mitochondrial imbalance in ALS.
  Xinxin Zuo, Jie Zhou, Yinming Li, Kai Wu, Zonggui Chen, Zhiwei Luo, Xiaorong Zhang, Yi Liang, Miguel A Esteban, Yu Zhou, Xiang-Dong Fu.
  
  Nature Structural & Molecular Biology 2021 Feb; 28(2):132.
  
  Application：WB-Ce, Mouse, N2a cells .
- MiR-378a-3p Is Critical for Burkitt Lymphoma Cell Growth.
  Fubiao Niu, Agnieszka Dzikiewicz-Krawczyk, Jasper Koerts, Debora de Jong, Laura Wijenberg, Margot Fernandez Hernandez, Izabella Slezak-Prochazka, Melanie Winkle, Wierd Kooistra, Tineke van der Sluis, Bea Rutgers, Miente Martijn Terpstra, Klaas Kok, Joost Kluiver, Anke van den Berg.
  
  Cancers 2020 Nov; 12(12):3546.
  
  Application：IP, WB-Tr, Human, ST486 cells.
- The nuclear and cytoplasmic roles of miR-320 in non-alcoholic fatty liver disease.
  Jiabing Zhan, Huizhen Lv, Beibei Dai, Shuai Yuan, Jiahui Fan, Yanru Zhao, Zhongwei Yin, Dao Wen Wang, Chen Chen, Huaping Li.
  
  Aging 2020 Nov; 12(21):22019.
  
  Application：ChIP, WB-Tr, Human, L02 cells.
- Oligodendrocyte-specific Argonaute profiling identifies microRNAs associated with experimental autoimmune encephalomyelitis.
  Qin Ma, Atsuko Matsunaga, Brenda Ho, Jorge R Oksenberg, Alessandro Didonna.
  
  Journal of Neuroinflammation 2020 Oct; 17(1):297.
  
  Application：IP, WB-Ti, Mouse, Mouse brain, Mouse central nervous system tissues, Mouse spinal cord.
- MiR-221-3p targets Hif-1α to inhibit angiogenesis in heart failure.
  Yuying Li, Chenghui Yan, Jiahui Fan, Zhiwei Hou, Yaling Han.
  
  Laboratory Investigation; a Journal of Technical Methods and Pathology 2021 Jan; 101(1):104.
  
  Application：IP, WB-Tr, Human, HUVECs.
- High-Throughput Identification of MiR-145 Targets in Human Articular Chondrocytes.
  Aida Martinez-Sanchez, Stefano Lazzarano, Eshita Sharma, Helen Lockstone, Christopher L Murphy.
  
  Life (Basel, Switzerland) 2020 May; 10(5):58.
  
  Application：IP, WB-Tr, Human, Human articular chondrocytes, Human chondrocytes.
- hsa-miR-20b-5p and hsa-miR-363-3p Affect Expression of PTEN and BIM Tumor Suppressor Genes and Modulate Survival of T-ALL Cells In Vitro.
  Monika Drobna, Bronisława Szarzyńska, Roman Jaksik, Łukasz Sędek, Anna Kuchmiy, Tom Taghon, Pieter Van Vlierberghe, Tomasz Szczepański, Michał Witt, Małgorzata Dawidowska.
  
  Cells 2020 May; 9(5):E1137.
  
  Application：IP, Human, DND-41 cells.
- Retrograde trafficking of Argonaute 2 acts as a rate-limiting step for de novo miRNP formation on endoplasmic reticulum-attached polysomes in mammalian cells.
  Bose M, Chatterjee S, Chakrabarty Y, Barman B, Bhattacharyya SN.
  
  Life Science Alliance 2020 Feb; 3(2):e201800161.
  
  Application：IP, WB-Tr, Human, HEK 293 cells.
- Identification of ncRNA-Mediated Functions of Nucleus-Localized miR-320 in Cardiomyocytes.
  Li H, Zhan J, Zhao Y, Fan J, Yuan S, Yin Z, Dai B, Chen C, Wang DW.
  
  Molecular Therapy. Nucleic Acids 2020 Mar; 19:132.
  
  Application：ChIP-Seq, RIP-Seq, Mouse, Rat, H9c2, HL-1 cells, Mouse hearts.
- Nuclear miR-665 aggravates heart failure via suppressing phosphatase and tensin homolog transcription.
  Fan J, Zhang X, Nie X, Li H, Yuan S, Dai B, Zhan J, Wen Z, Jiang J, Chen C, Wang D.
  
  Science China. Life sciences 2020 May; 63(5):724.
  
  Application：ChIP, Rat, Rat cardiomyocytes.
- UPF1/SMG7-dependent microRNA-mediated gene regulation.
  Park J, Seo JW, Ahn N, Park S, Hwang J, Nam JW.
  
  Nature Communications 2019 Sep; 10(1):4181.
  
  Application：WB-Tr, Human, HeLa cells.
- NMDAR mediated translation at the synapse is regulated by MOV10 and FMRP.
  Kute PM, Ramakrishna S, Neelagandan N, Chattarji S, Muddashetty RS.
  
  Molecular Brain 2019 Jul; 12(1):65.
  
  Application：IP, WB-Ti, WB-Tr, Mouse, Rat, Neuro2a cells, Rat cortical synaptoneurosomes.
- MicroRNA inhibition upregulates hippocampal A-type potassium current and reduces seizure frequency in a mouse model of epilepsy.
  Tiwari D, Brager DH, Rymer JK, Bunk AT, White AR, Elsayed NA, Krzeski JC, Snider A, Schroeder Carter LM, Danzer SC, Gross C.
  
  Neurobiology of Disease 2019 Oct; 130:104508.
  
  Application：IP, Mouse, Mouse hippocampus.
- Overexpression miR-24-3p repressed Bim expression to confer tamoxifen resistance in breast cancer.
  Han X, Li Q, Liu C, Wang C, Li Y.
  
  Journal of Cellular Biochemistry 2019 Aug; 120(8):12966.
  
  Application：IP, WB, Human, MCF-7 cells.
- A novel class of microRNA-recognition elements that function only within open reading frames.
  Zhang K, Zhang X, Cai Z, Zhou J, Cao R, Zhao Y, Chen Z, Wang D, Ruan W, Zhao Q, Liu G, Xue Y, Qin Y, Zhou B, Wu L, Nilsen T, Zhou Y, Fu XD.
  
  Nature Structural & Molecular Biology 2018 Nov; 25(11):1019.
  
  Application：WB-Tr, Human, HeLa cells.
- MiR-21 protected against diabetic cardiomyopathy induced diastolic dysfunction by targeting gelsolin.
  Dai B, Li H, Fan J, Zhao Y, Yin Z, Nie X, Wang DW, Chen C.
  
  Cardiovascular Diabetology 2018 Sep; 17(1):123.
  
  Application：IP, Human, Mouse, Rat, H9c2, HL-1 cells, Human cardiac myocytes.
- miR-217 Promotes Cardiac Hypertrophy and Dysfunction by Targeting PTEN.
  Nie X, Fan J, Li H, Yin Z, Zhao Y, Dai B, Dong N, Chen C, Wang DW.
  
  Molecular Therapy. Nucleic Acids 2018 Sep; 12:254.
  
  Application：IP, WB-Tr, Rat, H9c2 cells.
- Argonaute 2 RNA Immunoprecipitation Reveals Distinct miRNA Targetomes of Primary Burkitt Lymphoma Tumors and Normal B Cells.
  Dzikiewicz-Krawczyk A, Diepstra A, Rutgers B, Kortman G, de Jong D, Koerts J, Bulthuis M, van der Sluis T, Seitz A, Visser L, Kok K, Kluiver J, van den Berg A.
  
  The American Journal of Pathology 2018 May; 188(5):1289.
  
  Application：IP, Human, Primary BL tumors, tonsil CD19+ B cells.
- Site-Specific Modification Using the 2'-Methoxyethyl Group Improves the Specificity and Activity of siRNAs.
  Song X, Wang X, Ma Y, Liang Z, Yang Z, Cao H.
  
  Molecular Therapy. Nucleic Acids 2017 Dec; 9:242.
  
  Application：RIP-A, Human, HEK 293 cells.
- Identification of transforming growth factor-beta-regulated microRNAs and the microRNA-targetomes in primary lung fibroblasts.
  Ong J, Timens W, Rajendran V, Algra A, Spira A, Lenburg ME, Campbell JD, van den Berge M, Postma DS, van den Berg A, Kluiver J, Brandsma CA.
  
  PLoS One 2017 Sep; 12(9):e0183815.
  
  Application：IP, WB, Human, Human primary parenchymal lung fibroblasts.
- Glucocorticoids downregulate TLR4 signaling activity via its direct targeting by miR-511-5p.
  Curtale G, Renzi TA, Drufuca L, Rubino M, Locati M.
  
  European Journal of Immunology 2017 Aug; [Epub].
  
  Application：IP, Human, THP-1 cells, Human monocytes.
- Multivalent Recruitment of Human Argonaute by GW182.
  Elkayam E, Faehnle CR, Morales M, Sun J, Li H, Joshua-Tor L.
  
  Molecular Cell 2017 Aug; 67(4):646.
  
  Application：WB, Purified hAgo2-RNA complex.
- miR-24-3p Is Overexpressed in Hodgkin Lymphoma and Protects Hodgkin and Reed-Sternberg Cells from Apoptosis.
  Yuan Y, Kluiver J, Koerts J, de Jong D, Rutgers B, Razak FRA, Terpstra M, Plaat B, Nolte IM, Diepstra A, Visser L, Kok K, van den Berg A.
  
  Amino Acids 2017 Apr; 187(6):1343.
  
  Application：IP, WB, Human, L428, L540, L1236, KM-H2 cells.
- Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6.
  Huang JH, Ku WC, Chen YC, Chang YL, Chu CY.
  
  Scientific Reports 2017 Feb; 7:42853.
  
  Application：WB-Tr, Human, HeLa cells.
- Spatio-temporal uncoupling of miRNA-mediated translational repression and target RNA degradation controls miRNP recycling in mammalian cells.
  Bose M, Barman B, Goswami A, Bhattacharyya SN.
  
  Molecular and Cellular Biology 2016 Nov; [Epub].
  
  Application：WB-Tr, Human, HeLa, HEK 293 cells.
- LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion in cholangiocarcinoma through a ceRNA manner.
  Wang WT, Ye H, Wei PP, Han BW, He B, Chen ZH, Chen YQ.
  
  J Hematol Oncol 2016 Nov; 9(1):117.
  
  Application：IP-WB, Human, QBC939 human cholangiocarcinoma cells, RBE human cholangiocarcinoma cells.
- Infected erythrocyte-derived extracellular vesicles alter vascular function via regulatory Ago2-miRNA complexes in malaria.
  Mantel PY, Hjelmqvist D, Walch M, Kharoubi-Hess S, Nilsson S, Ravel D, Ribeiro M, Grüring C, Ma S, Padmanabhan P, Trachtenberg A, Ankarklev J, Brancucci NM, Huttenhower C, Duraisingh MT, Ghiran I, Kuo WP, Filgueira L, Martinelli R, Marti M.
  
  Nature Communications 2016 Oct; 7:12727.
  
  Application：WB, IF, Human, Human RBC.
- Derepression of miRNA-138 contributes to loss of the human articular chondrocyte phenotype.
  Seidl CI, Martinez-Sanchez A, Murphy CL.
  
  Arthritis & Rheumatology 2016 Feb; 68(2):398.
  
  Application：IP, Human, HACs.
- MiR-24-3p Promotes Porcine Reproductive and Respiratory Syndrome Virus Replication through Suppression of Heme Oxygenase-1 Expression.
  Xiao S, Wang X, Ni H, Li N, Zhang A, Liu H, Pu F, Xu L, Gao J, Zhao Q, Mu Y, Wang C, Sun Y, Du T, Xu X, Zhang G, Hiscox JA, Goodfellow IG, Zhou EM.
  
  Journal of Virology 2015 Apr; 89(8):4494.
  
  Application：Co-IP, African green monkey, MARC cells.
- The miR-424/503 cluster reduces CDC25A expression during cell cycle arrest imposed by TGFβ in mammary epithelial cells.
  Llobet-Navas D, Rodriguez-Barrueco R, de la Iglesia-Vicente J, Olivan M, Castro V, Saucedo-Cuevas L, Marshall N, Putcha P, Castillo-Martin M, Bardot E, Ezhkova E, Iavarone A, Cordon-Cardo C, Silva JM.
  
  Molecular and Cellular Biology 2014 Dec; 34(23):4216.
  
  Application：IP, Human, MCF-10A cells.
- Small RNA Sequence Analysis of Adenovirus VA RNA-Derived MiRNAs Reveals an Unexpected Serotype-Specific Difference in Structure and Abundance.
  Kamel W, Segerman B, Punga T, Akusjarvi G.
  
  PLoS One 2014 Aug; 9(8):e105746.
  
  Application：WB-Tr, Human, HeLa cells.
- MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.
  Zhang X, Zuo X, Yang B, Li Z, Xue Y, Zhou Y, Huang J, Zhao X, Zhou J, Yan Y, Zhang H, Guo P, Sun H, Guo L, Zhang Y, Fu XD.
  
  Cell 2014 Jul; 158(3):607.
  
  Application：WB-Ce, WB-Tr, Mouse, C2C12 cells, MEFs.
- Characterisation and Comparison of Lactating Mouse and Bovine Mammary Gland miRNomes.
  Le Guillou S, Marthey S, Laloe D, Laubier J, Mobuchon L, Leroux C, Le Provost F.
  
  PloS One 2014 Mar; 9(3):e91938.
  
  Application：IP, WB, Mouse, Mammary glands.
- NMDA receptor-dependent regulation of miRNA expression and association with Argonaute during LTP in vivo.
  Pai B, Siripornmongcolchai T, Berentsen B, Pakzad A, Vieuille C, Pallesen S, Pajak M, Simpson TI, Armstrong JD, Wibrand K, Bramham CR.
  
  Frontiers in Cellular Neuroscience 2014 Jan; 7:285.
  
  Application：IP, Recombinant protein.
- MicroRNA-23 inhibits PRRSV replication by directly targeting PRRSV RNA and possibly by upregulating type I interferons.
  Zhang Q, Guo XK, Gao L, Huang C, Li N, Jia X, Liue W, Feng WH.
  
  Virology 2014 Feb; 450-451:182.
  
  Application：IP, Pig, PAM cells.
- Identification of axon-enriched microRNAs localized in growth cones of cortical neurons.
  Sasaki Y, Gross C, Xing L, Goshima Y, Bassell GJ.
  
  Developmental Neurobiology 2014 Mar; 74(3):397.
  
  Application：IP, Mouse, Mouse cortex, Mouse hippocampus.
- Comprehensive Analysis of MicroRNA (miRNA) Targets in Breast Cancer Cells.
  Fan M, Krutilina R, Sun J, Sethuraman A, Yang CH, Wu ZH, Yue J, Pfeffer LM.
  
  The Journal of Biological Chemistry 2013 Sep; 288(38):27480.
  
  Application：IP, IP-WB, Human, MCF-7, MDA-MB-231 cells.
- Remodeling of Ago2-mRNA interactions upon cellular stress reflects miRNA complementarity and correlates with altered translation rates.
  Karginov FV, Hannon GJ.
  
  Genes & Development 2013 Jul; 27(14):1624.
  
  Application：IF, WB, Human, HeLa cells.
- Negative regulation of Toll-like receptor 4 signaling by IL-10-dependent microRNA-146b.
  Curtale G, Mirolo M, Renzi TA, Rossato M, Bazzoni F, Locati M.
  
  PNAS 2013 Jul; 110(28):11499.
  
  Application：RIP-A, Human, Monocytes, THP-1 cells.
- Single modification at position 14 of siRNA strand abolishes its gene-silencing activity by decreasing both RISC loading and target degradation.
  Zheng J, Zhang L, Zhang J, Wang X, Ye K, Xi Z, Du Q, Liang Z.
  
  FASEB Journal 2013 Oct; 27(10):4017.
  
  Application：RIP-A, Human, HEK 293 cells.
- Activated platelets can deliver mRNA regulatory Ago2{middle dot}microRNA complexes to endothelial cells via microparticles.
  Laffont B, Corduan A, Ple H, Duchez AC, Cloutier N, Boilard E, Provost P.
  
  Blood 2013 Jul; 122(2):253.
  
  Application：IP, WB-Ce, Human, Platelets.
- The microRNA-342-5p Fosters Inflammatory Macrophage Activation Through an Akt1- and microRNA-155-Dependent Pathway During Atherosclerosis.
  Wei Y, Nazari-Jahantigh M, Chan L, Zhu M, Heyll K, Corbalan-Campos J, Hartmann P, Thiemann A, Weber C, Schober A.
  
  Circulation 2013 Apr; 127(15):1609.
  
  Application：IP, Mouse, Macrophages.
- Loss of miR-125b-1 contributes to head and neck cancer development by dysregulating TACSTD2 and MAPK pathway.
  Nakanishi H, Taccioli C, Palatini J, Fernandez-Cymering C, Cui R, Kim T, Volinia S, Croce CM.
  
  Oncogene 2014 Feb; 33(6):702.
  
  Application：IP, Human, FaDu cells.
- Increasing expression of microRNA 181 inhibits porcine reproductive and respiratory syndrome virus replication and has implications for controlling virus infection.
  Guo XK, Zhang Q, Gao L, Li N, Chen XX, Feng WH.
  
  Journal of Virology 2013 Jan; 87(2):1159.
  
  Application：WB-Ce, Pig, Porcine alveolar macrophages.
- Direct Conversion of Fibroblasts to Neurons by Reprogramming PTB-Regulated MicroRNA Circuits.
  Xue Y, Ouyang K, Huang J, Zhou Y, Ouyang H, Li H, Wang G, Wu Q, Wei C, Bi Y, Jiang L, Cai Z, Sun H, Zhang K, Zhang Y, Chen J, Fu XD.
  
  Cell 2013 Jan; 152(1-2):82.
  
  Application：WB, Human, HeLa .
- siRNA has greatly elevated mismatch tolerance at 3'-UTR sites.
  Wei N, Zhang L, Huang H, Chen Y, Zheng J, Zhou X, Yi F, Du Q, Liang Z.
  
  PLoS One 2012 Nov; 7(11):e49309.
  
  Application：WB-Tr, Human, HEK 293 cells.
- The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function.
  Loedige I, Gaidatzis D, Sack R, Meister G, Filipowicz W.
  
  Nucleic Acids Research 2013 Jan; 41(1):518.
  
  Application：WB, Human, HEK 293 cells.
- MicroRNA-155 promotes atherosclerosis by repressing Bcl6 in macrophages.
  Nazari-Jahantigh M, Wei Y, Noels H, Akhtar S, Zhou Z, Koenen RR, Heyll K, Gremse F, Kiessling F, Grommes J, Weber C, Schober A.
  
  The Journal of Clinical Investigation 2012 Nov; 122(11):4190.
  
  Application：IP, Mouse, Mouse macrophages.
- The p38/MK2-driven exchange between tristetraprolin and HuR regulates AU-rich element-dependent translation.
  Tiedje C, Ronkina N, Tehrani M, Dhamija S, Laass K, Holtmann H, Kotlyarov A, Gaestel M.
  
  PLoS Genetics 2012 Sep; 8(9):e1002977.
  
  Application：WB, Monkey, MK2 cells.
- HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly.
  Reed JC, Molter B, Geary CD, McNevin J, McElrath J, Giri S, Klein KC, Lingappa JR.
  
  The Journal of Cell Biology 2012 Aug; 198(3):439.
  
  Application：WB-Ce, Human, H9-HIV cells.
- Generation of miRNA sponge constructs.
  Kluiver J, Slezak-Prochazka I, Smigielska-Czepiel K, Halsema N, Kroesen BJ, van den Berg A.
  
  Methods 2012 Oct; 58(2):113.
  
  Application：IP.
- MicroRNAs regulate p21(Waf1/Cip1) protein expression and the DNA damage response in human embryonic stem cells.
  Dolezalova D, Mraz M, Barta T, Plevova K, Vinarsky V, Holubcova Z, Jaros J, Dvorak P, Pospisilova S, Hampl A.
  
  Stem Cells 2012 Jul; 30(7):1362.
  
  Application：WB-Ce, Human, Human embryonic stem cells.
- A pathogenic mechanism in Huntington's disease involves small CAG-repeated RNAs with neurotoxic activity.
  Bañez-Coronel M, Porta S, Kagerbauer B, Mateu-Huertas E, Pantano L, Ferrer I, Guzmán M, Estivill X, Martí E.
  
  PLoS Genetics 2012 Feb; 8(2):e1002481.
  
  Application：WB-Tr, Human, SH-SY5Y cells.
- Regulation of Cyclin T1 and HIV-1 Replication by MicroRNAs in Resting CD4+ T Lymphocytes.
  Chiang K, Sung TL, Rice AP.
  
  Journal of Virology 2011 Dec; 86(6):3244.
  
  Application：IP-WB, Human, Jurkat cells.
- Regulation of human chondrocyte function through direct inhibition of cartilage master-regulator SOX9 by miRNA-145.
  Martinez-Sanchez A, Dudek KA, Murphy CL.
  
  The Journal of Biological Chemistry 2012 Jan; 287(2):916.
  
  Application：IP, Human, Articular chondrocytes.
- Fragile X Related Protein 1 Clusters with Ribosomes and Messenger RNAs at a Subset of Dendritic Spines in the Mouse Hippocampus.
  Cook D, Del Rayo Sanchez-Carbente M, Lachance C, Radzioch D, Tremblay S, Khandjian EW, Desgroseillers L, Murai KK.
  
  PLoS One 2011 Oct; 6(10):e26120.
  
  Application：IF, Mouse, Hippocampus, Neurons.
- Characterization of extracellular circulating microRNA.
  Turchinovich A, Weiz L, Langheinz A, Burwinkel B.
  
  Nucleic Acids Research 2011 Sep; 39(16):7223.
  
  Application：IP, WB-Tr, Human, MCF-7 cells, Plasma, Retinates.
- Limiting Ago protein restricts RNAi and microRNA biogenesis during early development in Xenopus laevis.
  Lund E, Sheets MD, Imboden SB, Dahlberg JE.
  
  Genes & Development 2011 Jun; 25(11):1121.
  
  Application：WB-Ti, WB-Tr, Xenopus laevis, Embryos, Oocytes.
- HuR-dependent loading of miRNA RISC to the mRNA encoding the Ras-related small GTPase RhoB controls its translation during UV-induced apoptosis.
  Glorian V, Maillot G, Poles S, Iacovoni JS, Favre G, Vagner S.
  
  Cell Death and Differentiation 2011 Nov; 18(11):1692.
  
  Application：IP, Human, Keratinocyte cells.
- Viral RNAi Suppressor Reversibly Binds siRNA to Outcompete Dicer and RISC via Multiple Turnover.
  Rawlings RA, Krishnan V, Walter NG.
  
  J Mol Biol 2011 Feb; 408:262.
  
  Application：WB-Ce, Human, HeLa cells.
- The QKI-6 RNA Binding Protein Localizes with the MBP mRNAs in Stress Granules of Glial Cells.
  Wang Y, Lacroix G, Haines J, Doukhanine E, Almazan G, Richard S.
  
  PLoS One 2010 Sep; 5(9):e12824.
  
  Application：IF, WB-Ce, WB-Tr, Human, Rat, Rat oligodendrocytes, U343 cells.
- A dicer-independent miRNA biogenesis pathway that requires Ago catalysis.
  Cheloufi S, Dos Santos CO, Chong MM, Hannon GJ.
  
  Nature 2010 Jun; 465(7298):584.
  
  Application：IP, WB, Mouse, Embryonic stem cells, Livers.
- P-Body Loss Is Concomitant with Formation of a Messenger RNA Storage Domain in Mouse Oocytes.
  Flemr M, Ma J, Schultz RM, Svoboda P.
  
  Biology of Reproduction 2010 May; 82(5):1008.
  
  Application：IF, Mouse, Oocytes.
- RNA binding motif protein 4 translocates to cytoplasmic granules and suppresses translation via Argonaute2 during muscle cell differentiation.
  Lin JC, Tarn WY.
  
  The Journal of Biological Chemistry 2009 Sep; 284(50):34658.
  
  Application：IF, WB, Mouse, C2C12, HEK 293 cells.
- A high throughput experimental approach to identify miRNA targets in human cells.
  Tan LP, Seinen E, Duns G, de Jong D, Sibon OC, Poppema S, Kroesen BJ, Kok K, van den Berg A.
  
  Nucleic Acids Research 2009 Nov; 37(20):e137.
  
  Application：IP, Human, L428, L1236 cells.
- Existence of a microRNA pathway in anucleate platelets.
  Landry P, Plante I, Ouellet DL, Perron MP, Rousseau G, Provost P.
  
  Nature Structural & Molecular Biology 2009 Sep; 16(9):961.
  
  Application：IF, WB, Human, Megakaryocytes, Platelets.
- Dynamic Interaction between P-Bodies and Transport Ribonucleoprotein Particles in Dendrites of Mature Hippocampal Neurons.
  Zeitelhofer M, Karra D, Macchi P, Tolino M, Thomas S, Schwarz M, Kiebler M, Dahm R.
  
  Journal of Neuroscience 2008 Jul; 28(30):7555.
  
  Application：IF, WB, Rat, Embryonic day 17 (E17) rat hippocampal neurons.
- Argonaute2 Is Essential for Mammalian Gastrulation and Proper Mesoderm Formation.
  Alisch RS, Jin P, Epstein M, Caspary T, Warren ST.
  
  PLoS Genetics 2007 Dec; 3(12):e227.
  
  Application：WB-Ti, Mouse, Mouse embryo.
- Reduced levels of Ago2 expression result in increased siRNA competition in mammalian cells.
  Vickers TA, Lima WF, Nichols JG, Crooke ST.
  
  Nucleic Acids Research 2007 Sep; 35(19):6598.
  
  Application：WB-Ce, Human, HeLa, T47D, U87 cells.
- Ribonuclease Dicer Cleaves Triplet Repeat Hairpins into Shorter Repeats that Silence Specific Targets.
  Krol J, Fiszer A, Mykowska A, Sobczak K, de Mezer M, Krzyzosiak WJ.
  
  Molecular Cell 2007 Feb; 25(4):575.
  
  Application：WB, Human, Myotonic dystrophy type 1 (DM1) fibroblast cells.