EIF2AK1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
EIF2AK1 transfected lysate.
Western Blot
Lane 1: EIF2AK1 transfected lysate ( 71.10 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-EIF2AK1 full-length
Host
Human
Theoretical MW (kDa)
71.1
Interspecies Antigen Sequence
Mouse (82); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EIF2AK1 antibody (H00027102-D01P) by Western Blots.
SDS-PAGE Gel
EIF2AK1 transfected lysate.
Western Blot
Lane 1: EIF2AK1 transfected lysate ( 71.10 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — EIF2AK1
Entrez GeneID
27102GeneBank Accession#
NM_014413.2Protein Accession#
NP_055228.2Gene Name
EIF2AK1
Gene Alias
HCR, HRI, KIAA1369
Gene Description
eukaryotic translation initiation factor 2-alpha kinase 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene acts at the level of translation initiation to downregulate protein synthesis in response to stress. The encoded protein is a kinase that can be inactivated by hemin. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
heme regulated initiation factor 2 alpha kinase|heme sensitive initiation factor 2a kinase|heme-regulated inhibitor|heme-regulated initiation factor 2-alpha kinase|heme-regulated repressor
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Interactome
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Disease
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