NPHP3 monoclonal antibody (M05), clone 3B1
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NPHP3.
Immunogen
NPHP3 (NP_694972.3, 106 a.a. ~ 205 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
NQELLSMGRREAKLDTENKRLRAELQALQKTYQKILREKESALEAKYQAMERAATFEHDRDKVKRQFKIFRETKENEIQDLLRAKRELESKLQRLQAQGI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (96)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NPHP3 monoclonal antibody (M05), clone 3B1. Western Blot analysis of NPHP3 expression in HepG2.Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NPHP3 is 3 ng/ml as a capture antibody.ELISA
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Gene Info — NPHP3
Entrez GeneID
27031GeneBank Accession#
NM_153240Protein Accession#
NP_694972.3Gene Name
NPHP3
Gene Alias
DKFZp667K242, DKFZp781K1312, FLJ30691, FLJ36696, KIAA2000, MGC78666, NPH3
Gene Description
nephronophthisis 3 (adolescent)
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein containing a coiled-coil (CC) domain, a tubulin-tyrosine ligase (TTL) domain, and a tetratrico peptide repeat (TPR) domain. The encoded protein interacts with nephrocystin and may function in renal tubular development and function. Mutations in this gene are associated with nephronophthisis type 3. Multiple splice variants have been described but their full-length nature has not been determined. [provided by RefSeq
Other Designations
nephrocystin 3
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Interactome
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Disease
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