DNAI1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DNAI1 transfected lysate.
Western Blot
Lane 1: DNAI1 transfected lysate ( 77 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DNAI1 full-length
Host
Human
Theoretical MW (kDa)
77
Interspecies Antigen Sequence
Mouse (87); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DNAI1 antibody (H00027019-B01) by Western Blots.
SDS-PAGE Gel
DNAI1 transfected lysate.
Western Blot
Lane 1: DNAI1 transfected lysate ( 77 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DNAI1
Entrez GeneID
27019GeneBank Accession#
NM_012144.2Protein Accession#
NP_036276.1Gene Name
DNAI1
Gene Alias
CILD1, ICS, ICS1, MGC26204, PCD
Gene Description
dynein, axonemal, intermediate chain 1
Gene Ontology
HyperlinkGene Summary
The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. This gene encodes an intermediate chain dynein, belonging to the large family of motor proteins. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia (PCD) and Kartagener syndrome. [provided by RefSeq
Other Designations
OTTHUMP00000021280|dynein intermediate chain DNAI1|dynein, axonemal, intermediate polypeptide 1|immotile cilia syndrome 1
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Interactome
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Disease
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