PABPC1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PABPC1 transfected lysate.
Western Blot
Lane 1: PABPC1 transfected lysate ( 70.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PABPC1 full-length
Host
Human
Theoretical MW (kDa)
70.7
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PABPC1 antibody (H00026986-D01P) by Western Blots.
SDS-PAGE Gel
PABPC1 transfected lysate.
Western Blot
Lane 1: PABPC1 transfected lysate ( 70.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PABPC1
Entrez GeneID
26986GeneBank Accession#
NM_002568.3Protein Accession#
NP_002559.2Gene Name
PABPC1
Gene Alias
PAB1, PABP, PABP1, PABPC2, PABPL1
Gene Description
poly(A) binding protein, cytoplasmic 1
Omim ID
604679Gene Ontology
HyperlinkGene Summary
The poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.[supplied by OMIM
Other Designations
poly(A) binding protein, cytoplasmic 2|poly(A)-binding protein, cytoplasmic 2
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