DAZAP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DAZAP1 transfected lysate.
Western Blot
Lane 1: DAZAP1 transfected lysate ( 44.88 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DAZAP1 full-length
Host
Human
Theoretical MW (kDa)
44.88
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DAZAP1 antibody (H00026528-B01) by Western Blots.
SDS-PAGE Gel
DAZAP1 transfected lysate.
Western Blot
Lane 1: DAZAP1 transfected lysate ( 44.88 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DAZAP1
Entrez GeneID
26528GeneBank Accession#
NM_018959Protein Accession#
NP_061832Gene Name
DAZAP1
Gene Alias
MGC19907
Gene Description
DAZ associated protein 1
Omim ID
607430Gene Ontology
HyperlinkGene Summary
In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene. [provided by RefSeq
Other Designations
deleted in azoospermia associated protein 1
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