HEYL monoclonal antibody (M07), clone 1F6
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More Files
- More Functions
Western Blot (Cell lysate)
HEYL monoclonal antibody (M07), clone 1F6 Western Blot analysis of HEYL expression in HeLa ( Cat # L013V1 ).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to HEYL on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HEYL is approximately 1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to HEYL on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (37.51 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant HEYL.
Immunogen
HEYL (NP_055386, 221 a.a. ~ 327 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
PLRRATGIILPARRNVLPSRGASSTRRARPLERPATPVPVAPSSRAARSSHIAPLLQSSSPTPPGPTGSAAYVAVPTPNSSSPGPAGRPAGAMLYHSWVSEITEIGA
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (78); Rat (79)
Isotype
IgG
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.51 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
HEYL monoclonal antibody (M07), clone 1F6 Western Blot analysis of HEYL expression in HeLa ( Cat # L013V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to HEYL on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HEYL is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to HEYL on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — HEYL
Entrez GeneID
26508GeneBank Accession#
NM_014571Protein Accession#
NP_055386Gene Name
HEYL
Gene Alias
HRT3, MGC12623, bHLHb33
Gene Description
hairy/enhancer-of-split related with YRPW motif-like
Omim ID
609034Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq
Other Designations
HEY-like protein|OTTHUMP00000000536|hairy-related transcription factor 3
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Interactome
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Publication Reference
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Repression of androgen receptor activity by HEYL, a third member of the hairy/enhancer-of-split-related family of notch effectors.
Lavery DN, Villaronga MA, Walker MM, Patel A, Belandia B, Bevan CL.
J Biol Chem 2011 Mar; 286:17796.
Application：IF, IHC-P, WB-Ce, Human, RWPE-1, BPH-1, LNCaP cells, Prostate cancer.
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Repression of androgen receptor activity by HEYL, a third member of the hairy/enhancer-of-split-related family of notch effectors.
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