GAPDHS 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GAPDHS transfected lysate.
Western Blot
Lane 1: GAPDHS transfected lysate ( 44.99 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GAPDHS full-length
Host
Human
Theoretical MW (kDa)
44.99
Interspecies Antigen Sequence
Mouse (76); Rat (78)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GAPDHS antibody (H00026330-B01) by Western Blots.
SDS-PAGE Gel
GAPDHS transfected lysate.
Western Blot
Lane 1: GAPDHS transfected lysate ( 44.99 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GAPDHS
Entrez GeneID
26330GeneBank Accession#
NM_014364.3Protein Accession#
NP_055179.1Gene Name
GAPDHS
Gene Alias
GAPD2, GAPDH-2, GAPDS, HSD-35
Gene Description
glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
Omim ID
609169Gene Ontology
HyperlinkGene Summary
This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq
Other Designations
glyceraldehyde-3-phosphate dehydrogenase, testis-specific|spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
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Interactome
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