GAPDS (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human GAPDS full-length ORF ( AAH36373, 1 a.a. - 408 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
70.62
Interspecies Antigen Sequence
Mouse (76); Rat (78)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — GAPDHS
Entrez GeneID
26330GeneBank Accession#
BC036373Protein Accession#
AAH36373Gene Name
GAPDHS
Gene Alias
GAPD2, GAPDH-2, GAPDS, HSD-35
Gene Description
glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
Omim ID
609169Gene Ontology
HyperlinkGene Summary
This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility. [provided by RefSeq
Other Designations
glyceraldehyde-3-phosphate dehydrogenase, testis-specific|spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
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Interactome
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Disease
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Publication Reference
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Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm.
Margaryan H, Dorosh A, Capkova J, Manaskova-Postlerova P, Philimonenko A, Hozak P, Peknicova J.
Reproductive Biology and Endocrinology : RB&E 2015 Mar; 13(1):15.
Application：SDS-PAGE, WB-Ce, Human, Sperm cells.
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Characterization and possible function of glyceraldehyde-3-phosphate dehydrogenase-spermatogenic protein GAPDHS in mammalian sperm.
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