FBXO25 monoclonal antibody (M01), clone 2F1
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More Files
- More Functions
Western Blot (Cell lysate)
FBXO25 monoclonal antibody (M01), clone 2F1 Western Blot analysis of FBXO25 expression in COLO 320 HSR ( Cat # L020V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of FBXO25 expression in transfected 293T cell line by FBXO25 monoclonal antibody (M01), clone 2F1.
Lane 1: FBXO25 transfected lysate(43 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FBXO25 is approximately 3ng/ml as a capture antibody.
Western Blot detection against Immunogen (66.11 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant FBXO25.
Immunogen
FBXO25 (AAH50393, 1 a.a. ~ 367 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPFLGQDWRSPGWSWIKTEDGWKRCESCSQKLERENNHCNISHSIILNSEDGEIFNNEEHEYASKKRKKDHFRNDTNTQSFYREKWIYVHKESTKERHGYCTLGEAFNRLDFSSAIQDIRRFNYVVKLLQLIAKSQLTSLSGVAQKNYFNILDKIVQKVLDDHHNPRLIKDLLQDLSSTLCILIRGVGKSVLVGNINIWICRLETILAWQQQLQDLQMTKQVNNGLTLSDLPLHMLNNILYRFSDGWDIITLGQVTPTLYMLSEDRQLWKKLCQYHFAEKQFCRHLILSEKGHIEWKLMYFALQKHYPAKEQYGDTLHFCRHCSILFWKDYHLALLFKDSGHPCTAADPDSCFTPVSPQHFIDLFKF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (86); Rat (86)
Isotype
IgG2a kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (66.11 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
FBXO25 monoclonal antibody (M01), clone 2F1 Western Blot analysis of FBXO25 expression in COLO 320 HSR ( Cat # L020V1 ).Western Blot (Transfected lysate)
Western Blot analysis of FBXO25 expression in transfected 293T cell line by FBXO25 monoclonal antibody (M01), clone 2F1.
Lane 1: FBXO25 transfected lysate(43 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged FBXO25 is approximately 3ng/ml as a capture antibody.ELISA
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Gene Info — FBXO25
Entrez GeneID
26260GeneBank Accession#
BC050393Protein Accession#
AAH50393Gene Name
FBXO25
Gene Alias
FBX25, MGC20256, MGC51975
Gene Description
F-box protein 25
Omim ID
609098Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
Other Designations
F-box only protein 25|F-box protein Fbx25|OTTHUMP00000115399
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Interactome
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Disease
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