TIAM2 monoclonal antibody (M01), clone 3C1
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant TIAM2.
Immunogen
TIAM2 (NP_001010927, 527 a.a. ~ 624 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
DVHPEAEQQPGPESGEGQKGGEQPKLVRGHFCPIKRKANSTKRDRGTLLKAQIRHQSLDSQSENATIDLNSVLEREFSVQSLTSVVSEECFYETESHG
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.52 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of TIAM2 expression in transfected 293T cell line by TIAM2 monoclonal antibody (M01), clone 3C1.
Lane 1: TIAM2 transfected lysate(70.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TIAM2 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to TIAM2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — TIAM2
Entrez GeneID
26230GeneBank Accession#
NM_001010927Protein Accession#
NP_001010927Gene Name
TIAM2
Gene Alias
FLJ41865, STEF
Gene Description
T-cell lymphoma invasion and metastasis 2
Omim ID
604709Gene Ontology
HyperlinkGene Summary
This gene encodes a guanine nucleotide exchange factor. A highly similar mouse protein specifically activates ras-related C3 botulinum substrate 1, converting this Rho-like guanosine triphosphatase (GTPase) from a guanosine diphosphate-bound inactive state to a guanosine triphosphate-bound active state. The encoded protein may play a role in neural cell development. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000040111|SIF and TIAM1-like exchange factor
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Interactome
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Disease
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