PHGDH 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PHGDH transfected lysate.
Western Blot
Lane 1: PHGDH transfected lysate ( 56.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PHGDH full-length
Host
Human
Theoretical MW (kDa)
56.7
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PHGDH antibody (H00026227-B01) by Western Blots.
SDS-PAGE Gel
PHGDH transfected lysate.
Western Blot
Lane 1: PHGDH transfected lysate ( 56.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PHGDH
Entrez GeneID
26227GeneBank Accession#
NM_006623.2Protein Accession#
-Gene Name
PHGDH
Gene Alias
3-PGDH, 3PGDH, MGC3017, PDG, PGAD, PGD, PGDH, SERA
Gene Description
phosphoglycerate dehydrogenase
Gene Ontology
HyperlinkGene Summary
3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM
Other Designations
3-phosphoglycerate dehydrogenase|OTTHUMP00000013926
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Interactome
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Pathway
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Disease
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