PHGDH monoclonal antibody (M01J), clone 4A3-1D6
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant PHGDH.
This product is belong to Cell Culture Grade Antibody (CX Grade).Immunogen
PHGDH (AAH11262.1, 1 a.a. ~ 533 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGILGLGRIGREVATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Preparation Method
Cell Culture Production
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (84.37 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
PHGDH monoclonal antibody (M01J), clone 4A3-1D6. Western Blot analysis of PHGDH expression in human liver.Western Blot (Cell lysate)
PHGDH monoclonal antibody (M01J), clone 4A3-1D6. Western Blot analysis of PHGDH expression in MCF-7.Western Blot (Transfected lysate)
Western Blot analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody (M01J), clone 4A3-1D6.
Lane 1: PHGDH transfected lysate (Predicted MW: 56.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml]Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of PHGDH transfected lysate using anti-PHGDH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PHGDH MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PHGDH is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PHGDH
Entrez GeneID
26227GeneBank Accession#
BC011262Protein Accession#
AAH11262.1Gene Name
PHGDH
Gene Alias
3-PGDH, 3PGDH, MGC3017, PDG, PGAD, PGD, PGDH, SERA
Gene Description
phosphoglycerate dehydrogenase
Gene Ontology
HyperlinkGene Summary
3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM
Other Designations
3-phosphoglycerate dehydrogenase|OTTHUMP00000013926
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Interactome
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Pathway
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Disease
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