SPAG8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SPAG8 transfected lysate.
Western Blot
Lane 1: SPAG8 transfected lysate ( 55.22 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SPAG8 full-length
Host
Human
Theoretical MW (kDa)
55.22
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SPAG8 antibody (H00026206-B01) by Western Blots.
SDS-PAGE Gel
SPAG8 transfected lysate.
Western Blot
Lane 1: SPAG8 transfected lysate ( 55.22 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SPAG8
Entrez GeneID
26206GeneBank Accession#
BC019247.1Protein Accession#
AAH19247.1Gene Name
SPAG8
Gene Alias
BS-84, HSD-1, MGC26201, SMP1, SPAG3, hSMP-1
Gene Description
sperm associated antigen 8
Omim ID
605731Gene Ontology
HyperlinkGene Summary
The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq
Other Designations
OTTHUMP00000021352|sperm membrane protein 1
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