SENP3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SENP3 transfected lysate.
Western Blot
Lane 1: SENP3 transfected lysate ( 65 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SENP3 full-length
Host
Human
Theoretical MW (kDa)
65
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SENP3 antibody (H00026168-D01P) by Western Blots.
SDS-PAGE Gel
SENP3 transfected lysate.
Western Blot
Lane 1: SENP3 transfected lysate ( 65 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SENP3
Entrez GeneID
26168GeneBank Accession#
NM_015670.4Protein Accession#
NP_056485.2Gene Name
SENP3
Gene Alias
DKFZp586K0919, DKFZp762A152, SMT3IP1, SSP3
Gene Description
SUMO1/sentrin/SMT3 specific peptidase 3
Gene Ontology
HyperlinkGene Summary
The reversible posttranslational modification of proteins by the addition of small ubiquitin-like SUMO proteins (see SUMO1; MIM 601912) is required for numerous biologic processes. SUMO-specific proteases, such as SENP3, are responsible for the initial processing of SUMO precursors to generate a C-terminal diglycine motif required for the conjugation reaction. They also have isopeptidase activity for the removal of SUMO from high molecular mass SUMO conjugates (Di Bacco et al., 2006 [PubMed 16738315]).[supplied by OMIM
Other Designations
SUMO1/sentrin/SMT3 specific protease 3|sentrin/SUMO-specific protease 3
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Interactome
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Disease
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