GGA1 monoclonal antibody (M02), clone 1G8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of GGA1 expression in transfected 293T cell line by GGA1 monoclonal antibody (M02), clone 1G8.
Lane 1: GGA1 transfected lysate(61.4 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GGA1 is approximately 0.1ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of GGA1 over-expressed 293 cell line, cotransfected with GGA1 Validated Chimera RNAi ( Cat # H00026088-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GGA1 monoclonal antibody (M02), clone 1G8 (Cat # H00026088-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (35.53 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GGA1.
Immunogen
GGA1 (AAH29388, 1 a.a. ~ 89 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEPAMEPETLEARINRATNPLNKELDWASINGFCEQLNEDFEGPPLATRLLAHKIQSPQEWEAIQALTVRRGEATIRPPPCDDTKGGQD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (71); Rat (61)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of GGA1 expression in transfected 293T cell line by GGA1 monoclonal antibody (M02), clone 1G8.
Lane 1: GGA1 transfected lysate(61.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GGA1 is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of GGA1 over-expressed 293 cell line, cotransfected with GGA1 Validated Chimera RNAi ( Cat # H00026088-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GGA1 monoclonal antibody (M02), clone 1G8 (Cat # H00026088-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — GGA1
Entrez GeneID
26088GeneBank Accession#
BC029388Protein Accession#
AAH29388Gene Name
GGA1
Gene Alias
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Gene Description
golgi associated, gamma adaptin ear containing, ARF binding protein 1
Omim ID
606004Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) protein family. Members of this family are ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ADP-ribosylation factor binding protein 1|OTTHUMP00000028975|OTTHUMP00000042200|gamma-adaptin related protein 1
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Interactome
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Pathway
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