APPL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
APPL1 transfected lysate.
Western Blot
Lane 1: APPL1 transfected lysate ( 79.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-APPL full-length
Host
Human
Theoretical MW (kDa)
79.7
Interspecies Antigen Sequence
Mouse (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-APPL antibody (H00026060-D01P) by Western Blots.
SDS-PAGE Gel
APPL1 transfected lysate.
Western Blot
Lane 1: APPL1 transfected lysate ( 79.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — APPL1
Entrez GeneID
26060GeneBank Accession#
NM_012096Protein Accession#
NP_036228.1Gene Name
APPL1
Gene Alias
APPL, DIP13alpha
Gene Description
adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Omim ID
604299Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq
Other Designations
AKT2 interactor|adaptor protein containing pH domain, PTB domain and leucine zipper motif 1|signaling adaptor protein DIP13alpha
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Interactome
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Pathway
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Disease
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