SH2B monoclonal antibody (M01), clone 2B9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of SH2B1 expression in transfected 293T cell line by SH2B1 monoclonal antibody (M01), clone 2B9.
Lane 1: SH2B1 transfected lysate(52.917 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SH2B is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to SH2B1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SH2B.
Immunogen
SH2B (AAH10704, 327 a.a. ~ 426 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GKAKHLRLSLNEEGQCRVQHLWFQSIFDMLEHFRVHPIPLESGGSSDVVLVSYVPSSQRQQGREQAGSHAGVCEGDGCHPDASCTLMPFGASDCVTDHLP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (93)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SH2B1 expression in transfected 293T cell line by SH2B1 monoclonal antibody (M01), clone 2B9.
Lane 1: SH2B1 transfected lysate(52.917 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SH2B is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SH2B1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — SH2B1
Entrez GeneID
25970GeneBank Accession#
BC010704Protein Accession#
AAH10704Gene Name
SH2B1
Gene Alias
DKFZp547G1110, FLJ30542, KIAA1299, SH2-B, SH2B
Gene Description
SH2B adaptor protein 1
Omim ID
608937Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the SH2-domain containing mediators family. The encoded protein mediates activation of various kinases and may function in cytokine and growth factor receptor signaling and cellular transformation. Alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
SH2 domain-containing putative adapter SH2-B|SH2-B alpha signaling protein|SH2-B gamma signaling protein|SH2-B homolog
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Interactome
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Pathway
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Disease
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