COBRA1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
COBRA1 transfected lysate.
Western Blot
Lane 1: COBRA1 transfected lysate ( 53.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-COBRA1 full-length
Host
Human
Theoretical MW (kDa)
53.9
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-COBRA1 antibody (H00025920-D01P) by Western Blots.
SDS-PAGE Gel
COBRA1 transfected lysate.
Western Blot
Lane 1: COBRA1 transfected lysate ( 53.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — COBRA1
Entrez GeneID
25920GeneBank Accession#
BC011892.1Protein Accession#
AAH11892.1Gene Name
COBRA1
Gene Alias
DKFZp586B0519, KIAA1182, NELF-B, NELFB
Gene Description
cofactor of BRCA1
Gene Ontology
HyperlinkGene Summary
NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM
Other Designations
negative elongation factor protein B
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