BRMS1 monoclonal antibody (M01), clone 2D4-2G11
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant BRMS1.
Immunogen
BRMS1 (AAH09834, 1 a.a. ~ 246 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MPVQPPSKDTEEMEAEGDSAAEMNGEEEESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQRSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKLLLYDTLQGELQERIQRLEEDRQSLDLSSEWWDDKLHARGSSRSWDSLPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKSDGP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (92)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (52.8 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
BRMS1 monoclonal antibody (M01), clone 2D4-2G11. Western Blot analysis of BRMS1 expression in human liver.Western Blot (Transfected lysate)
Western Blot analysis of BRMS1 expression in transfected 293T cell line by BRMS1 monoclonal antibody (M01), clone 2D4-2G11.
Lane 1: BRMS1 transfected lysate (Predicted MW: 28.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of BRMS1 transfected lysate using anti-BRMS1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BRMS1 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged BRMS1 is approximately 3ng/ml as a capture antibody.ELISA
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Gene Info — BRMS1
Entrez GeneID
25855GeneBank Accession#
BC009834Protein Accession#
AAH09834Gene Name
BRMS1
Gene Alias
DKFZp564A063
Gene Description
breast cancer metastasis suppressor 1
Omim ID
606259Gene Ontology
HyperlinkGene Summary
This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
breast cancer metastasis-suppressor 1
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Interactome
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Publication Reference
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Expression of the metastasis suppressor BRMS1 in uveal melanoma.
Ventura BV, Quezada C, Maloney SC, Fernandes BF, Antecka E, Martins C, Bakalian S, di Cesare S, Burnier MN Jr.
Ecancermedicalscience 2014 Mar; 8:410.
Application:IHC-P, WB-Ce, Human, 92.1, SP6.5, MKT-BR, OCM-1 cells, Uveal melanoma.
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BRMS1 Suppresses Lung Cancer Metastases through an E3 Ligase Function on Histone Acetyltransferase p300.
Liu Y, Mayo MW, Nagji AS, Hall EH, Shock LS, Xiao A, Stelow EB, Jones DR.
Cancer Research 2013 Feb; 73(4):1308.
Application:WB, Human, NCI-H157 cells, HEK 293T cells, H358 cells, H460 cells, A549 cells, H1299 cells.
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Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival.
Cui RX, Liu N, He QM, Li WF, Huang BJ, Sun Y, Tang LL, Chen M, Jiang N, Chen L, Yun JP, Zeng J, Guo Y, Wang HY, Ma J.
BMC Cancer 2012 Aug; 12(1):376.
Application:IHC-P, WB-Ti, Human, Human nasopharyngeal carcinoma.
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Breast Cancer Metastasis Suppressor 1 (BRMS1) is destabilized by the Cul3-SPOP E3 ubiquitin ligase complex.
Kim B, Nam HJ, Pyo KE, Jang MJ, Kim IS, Kim D, Boo K, Lee SH, Yoon JB, Baek SH, Kim JH.
Biochemical and Biophysical Research Communications 2011 Dec; 415(4):720.
Application:WB-Tr, Human, HEK 293T, MCF-7, MDA-MB-435 cells.
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BRMS1 transcriptional repression correlates with CpG island methylation and advanced pathologic stage in non-small cell lung cancer.
Nagji AS, Liu Y, Stelow EB, Stukenborg GJ, Jones DR.
The Journal of Pathology 2010 Jun; 221(2):229.
Application:IHC-P, WB-Ce, Human, A-549, H157, Human lung cancer, NHBE cells.
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Breast cancer metastasis suppressor 1 (BRMS1) suppresses metastasis and correlates with improved patient survival in non-small cell lung cancer.
Smith PW, Liu Y, Siefert SA, Moskaluk CA, Petroni GR, Jones DR.
Cancer Letters 2009 Apr; 276(2):196.
Application:WB, Human, NCI-H1299 cells.
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Proteomics-Based Strategy To Delineate the Molecular Mechanisms of the Metastasis Suppressor Gene BRMS1.
Rivera J, Megias D, Bravo J.
Journal of Proteome Research 2007 Sep; 6(10):4006.
Application:WB, Human, Melanoma cell line.
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Breast cancer metastasis suppressor 1 functions as a corepressor by enhancing HDAC1-mediated deacetylation of RelA/p65 and promoting apoptosis.
Liu Y, Smith PW, Jones DR.
Molecular and Cellular Biology 2006 Sep; 26(23):8683.
Application:IP, WB, Human, human lung cancer, A549, H358, H1299, H157, H460, HEK 293T cells.
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Expression of the metastasis suppressor BRMS1 in uveal melanoma.
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