GCA monoclonal antibody (M01), clone 2F5
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More Files
- More Functions
Western Blot (Cell lysate)
GCA monoclonal antibody (M01), clone 2F5 Western Blot analysis of GCA expression in MCF-7 ( Cat # L046V1 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to GCA on MCF-7 cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (49.61 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant GCA.
Immunogen
GCA (AAH05214, 1 a.a. ~ 217 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (81); Rat (80)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (49.61 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
GCA monoclonal antibody (M01), clone 2F5 Western Blot analysis of GCA expression in MCF-7 ( Cat # L046V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GCA is 0.03 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to GCA on MCF-7 cell . [antibody concentration 10 ug/ml] -
Gene Info — GCA
Entrez GeneID
25801GeneBank Accession#
BC005214Protein Accession#
AAH05214Gene Name
GCA
Gene Alias
GCL
Gene Description
grancalcin, EF-hand calcium binding protein
Omim ID
607030Gene Ontology
HyperlinkGene Summary
This gene product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation. [provided by RefSeq
Other Designations
grancalcin, EF-hand calcium-binding protein|grancalcin, penta-EF-hand protein
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Interactome
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Disease
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