PGLS monoclonal antibody (M01), clone 2D9
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More Files
- More Functions
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant PGLS.
Immunogen
PGLS (AAH14006, 1 a.a. ~ 258 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAAPAPGLISVFSSSQELGAALAQLVAQRAACCLAGARARFALGLSGGNLVSMLARELPAAVAPAGPASLARWTLGFCDERLVPFDHAESTYGLYRTHLLSRLPIPESQVITINPELPVEEAAEDYAKKLRQAFQGDSIPVFDLLILGVGPDGHTCSLFPDHPLLQEREKIVAPISDSPKPPPQRVTLTLPVLNAARTVIFVATGEGKAAVLKRILEDQEENPLPAALVQPHTGKLCWFLDEAAARLLTVPFEKHSTL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (88)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (54.12 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PGLS expression in transfected 293T cell line by PGLS monoclonal antibody (M01), clone 2D9.
Lane 1: PGLS transfected lysate(27.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of PGLS transfected lysate using anti-PGLS monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGLS MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PGLS is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PGLS on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — PGLS
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Interactome
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Pathway
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