RAD54B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RAD54B transfected lysate.
Western Blot
Lane 1: RAD54B transfected lysate ( 18.60 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RAD54B full-length
Host
Human
Theoretical MW (kDa)
18.6
Interspecies Antigen Sequence
Mouse (31)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RAD54B antibody (H00025788-D01P) by Western Blots.
SDS-PAGE Gel
RAD54B transfected lysate.
Western Blot
Lane 1: RAD54B transfected lysate ( 18.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RAD54B
Entrez GeneID
25788GeneBank Accession#
BC033710Protein Accession#
AAH33710.2Gene Name
RAD54B
Gene Alias
FSBP, RDH54
Gene Description
RAD54 homolog B (S. cerevisiae)
Omim ID
604289Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq
Other Designations
Fibrinogen silencer-binding protein|RAD54 homolog B|RAD54, S. cerevisiae, homolog of, B|fibrinogen silencer binding protein
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Interactome
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