RAD54B monoclonal antibody (M01), clone 4A7
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RAD54B.
Immunogen
RAD54B (AAH01965, 801 a.a. ~ 910 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
HIQFSVEELKNLFTLHESSDCVTHDLLDCECTGEEVHTGDSLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNLTDPFLERITENVSFIFQNITTQATGT
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (31)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
RAD54B monoclonal antibody (M01), clone 4A7 Western Blot analysis of RAD54B expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RAD54B is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to RAD54B on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — RAD54B
Entrez GeneID
25788GeneBank Accession#
BC001965Protein Accession#
AAH01965Gene Name
RAD54B
Gene Alias
FSBP, RDH54
Gene Description
RAD54 homolog B (S. cerevisiae)
Omim ID
604289Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer. [provided by RefSeq
Other Designations
Fibrinogen silencer-binding protein|RAD54 homolog B|RAD54, S. cerevisiae, homolog of, B|fibrinogen silencer binding protein
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