RASGRP3 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of RASGRP3 transfected lysate using rabbit polyclonal anti-RASGRP3 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-RASGRP3.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of RASGRP3 transfected lysate using rabbit polyclonal anti-RASGRP3 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse purified polyclonal anti-RASGRP3.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-RASGRP3 (300 ul)
2. Antibody pair for WB: mouse purified polyclonal anti-RASGRP3 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — RASGRP3
Entrez GeneID
25780Gene Name
RASGRP3
Gene Alias
GRP3, KIAA0846
Gene Description
RAS guanyl releasing protein 3 (calcium and DAG-regulated)
Omim ID
609531Gene Ontology
HyperlinkGene Summary
Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RASGRP3, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM
Other Designations
guanine nucleotide exchange factor for Rap1
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Interactome
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Pathway
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Disease
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