CARKL 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
CARKL transfected lysate.
Western Blot
Lane 1: CARKL transfected lysate ( 52.69 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CARKL full-length
Host
Human
Theoretical MW (kDa)
52.69
Interspecies Antigen Sequence
Mouse (87); Rat (86)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CARKL antibody (H00023729-B01) by Western Blots.
SDS-PAGE Gel
CARKL transfected lysate.
Western Blot
Lane 1: CARKL transfected lysate ( 52.69 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SHPK
Entrez GeneID
23729GeneBank Accession#
NM_013276.2Protein Accession#
NP_037408.2Gene Name
SHPK
Gene Alias
CARKL, FLJ32478, SHK
Gene Description
sedoheptulokinase
Omim ID
605060Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene has weak homology to several carbohydrate kinases, a class of proteins involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane. Sequence variation between this novel gene and known carbohydrate kinases suggests the possibility of a different substrate, cofactor or changes in kinetic properties distinguishing it from other carbohydrate kinases. The gene resides in a region commonly deleted in cystinosis patients, suggesting a role as a modifier for the cystinosis phenotype. The genomic region is also rich in Alu repetitive sequences, frequently involved in chromosomal rearrangements. [provided by RefSeq
Other Designations
carbohydrate kinase-like
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Interactome
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Pathway
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Disease
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