SPO11 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SPO11 transfected lysate.
Western Blot
Lane 1: SPO11 transfected lysate ( 40.4 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SPO11 full-length
Host
Human
Theoretical MW (kDa)
39.49
Interspecies Antigen Sequence
Mouse (73); Rat (80)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SPO11 antibody (H00023626-B01) by Western Blots.
SDS-PAGE Gel
SPO11 transfected lysate.
Western Blot
Lane 1: SPO11 transfected lysate ( 40.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SPO11
Entrez GeneID
23626GeneBank Accession#
NM_198265Protein Accession#
NP_937998Gene Name
SPO11
Gene Alias
MGC39953
Gene Description
SPO11 meiotic protein covalently bound to DSB homolog (S. cerevisiae)
Omim ID
605114Gene Ontology
HyperlinkGene Summary
Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described. [provided by RefSeq
Other Designations
OTTHUMP00000031360|OTTHUMP00000031361|SPO11 meiotic protein covalently bound to DSB-like|SPO11, meiotic protein covalently bound to DSB-like|meiotic recombination protein SPO11
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Interactome
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Disease
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