BACE1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
BACE1 transfected lysate.
Western Blot
Lane 1: BACE1 transfected lysate ( 52.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BACE1 full-length
Host
Human
Theoretical MW (kDa)
52.9
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BACE1 antibody (H00023621-D01P) by Western Blots.
SDS-PAGE Gel
BACE1 transfected lysate.
Western Blot
Lane 1: BACE1 transfected lysate ( 52.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BACE1
Entrez GeneID
23621GeneBank Accession#
NM_138972Protein Accession#
NP_620428.1Gene Name
BACE1
Gene Alias
ASP2, BACE, FLJ90568, HSPC104, KIAA1149
Gene Description
beta-site APP-cleaving enzyme 1
Omim ID
604252Gene Ontology
HyperlinkGene Summary
Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq
Other Designations
APP beta-secretase|aspartyl protease 2|beta-secretase|beta-site amyloid beta A4 precursor protein-cleaving enzyme|beta-site amyloid precursor protein cleaving enzyme|memapsin-2|membrane-associated aspartic protease 2|transmembrane aspartic proteinase Asp2
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Interactome
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Disease
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