ZIM2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ZIM2 transfected lysate.
Western Blot
Lane 1: ZIM2 transfected lysate ( 58.08 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ZIM2 full-length
Host
Human
Theoretical MW (kDa)
58.08
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ZIM2 antibody (H00023619-B01) by Western Blots.
SDS-PAGE Gel
ZIM2 transfected lysate.
Western Blot
Lane 1: ZIM2 transfected lysate ( 58.08 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ZIM2
Entrez GeneID
23619GeneBank Accession#
NM_015363.3Protein Accession#
NP_056178.3Gene Name
ZIM2
Gene Alias
ZNF656
Gene Description
zinc finger, imprinted 2
Gene Ontology
HyperlinkGene Summary
In human, ZIM2 and PEG3 (GeneID:5178) are two distinct genes that share a set of 5' exons and have a common promoter, and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect the shared exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. This is in contrast to mouse and cow, where ZIM2 and PEG3 genes do not share exons in common, and the imprinting status of ZIM2 is also not conserved amongst mammals. Additional 5' alternatively spliced transcripts encoding the same protein have been found for the human ZIM2 gene. [provided by RefSeq
Other Designations
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