ORC6L 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
ORC6L transfected lysate
Western Blot
Lane 1: ORC6L transfected lysate ( 27.83 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ORC6L full-length
Host
Human
Theoretical MW (kDa)
27.83
Interspecies Antigen Sequence
Mouse (74); Rat (70)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ORC6L antibody (H00023594-B01) by Western Blots.
SDS-PAGE Gel
ORC6L transfected lysate
Western Blot
Lane 1: ORC6L transfected lysate ( 27.83 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ORC6L
Entrez GeneID
23594GeneBank Accession#
BC063565Protein Accession#
AAH63565Gene Name
ORC6L
Gene Alias
ORC6
Gene Description
origin recognition complex, subunit 6 like (yeast)
Omim ID
607213Gene Ontology
HyperlinkGene Summary
The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. It has been shown that this protein and and ORC1L are loosely associated with the core complex consisting of ORC2L, -3L, -4L and -5L. Gene silencing studies with small interfering RNA demonstrated that this protein plays an essential role in coordinating chromosome replication and segregation with cytokinesis. [provided by RefSeq
Other Designations
origin recognition complex subunit 6|origin recognition complex, subunit 6 homolog-like
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Interactome
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Pathway
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