CLDN14 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CLDN14 full-length
Host
Human
Theoretical MW (kDa)
25.7
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GFI1 antibody (H00023562-D01) by Western Blots.
SDS-PAGE Gel
CLDN14 transfected lysate.
Western Blot
Lane 1: CLDN14 transfected lysate ( 25.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CLDN14
Entrez GeneID
23562GeneBank Accession#
NM_012130.2Protein Accession#
NP_036262.1Gene Name
CLDN14
Gene Alias
DFNB29
Gene Description
claudin 14
Omim ID
605608Gene Ontology
HyperlinkGene Summary
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. The encoded protein also binds specifically to the WW domain of Yes-associated protein. Defects in this gene are the cause of an autosomal recessive form of nonsyndromic sensorineural deafness. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000109045|OTTHUMP00000109046|OTTHUMP00000109047|OTTHUMP00000109048|OTTHUMP00000109049
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