GTPBP4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
SDS-PAGE Gel
GTPBP4 transfected lysate.
Western Blot
Lane 1: GTPBP4 transfected lysate ( 69.85 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GTPBP4 full-length
Host
Human
Theoretical MW (kDa)
69.85
Interspecies Antigen Sequence
Mouse (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GTPBP4 antibody (H00023560-B01) by Western Blots.
SDS-PAGE Gel
GTPBP4 transfected lysate.
Western Blot
Lane 1: GTPBP4 transfected lysate ( 69.85 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GTPBP4
Entrez GeneID
23560GeneBank Accession#
NM_012341.2Protein Accession#
-Gene Name
GTPBP4
Gene Alias
CRFG, FLJ10686, FLJ10690, FLJ39774, NGB, NOG1
Gene Description
GTP binding protein 4
Gene Ontology
HyperlinkGene Summary
GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target. [provided by RefSeq
Other Designations
G protein-binding protein CRFG|GTP-binding protein|chronic renal failure
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Interactome
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Disease
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