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Last updated: 2017/11/12

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PRAME 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00023532-T02
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-PRAME full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 57.9
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-PRAME antibody (H00023532-B01P) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00023532-T02
    PRAME transfected lysate.
    Western Blot
    QC Testing of H00023532-T02
    Lane 1: PRAME transfected lysate ( 57.90 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • PRAME
  • Gene Alias:
  • MAPE,OIP4
  • Gene Description:
  • preferentially expressed antigen in melanoma
  • Gene Summary:
  • This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq
  • Other Designations:
  • Opa-interacting protein OIP4,melanoma antigen preferentially expressed in tumors
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