PRAME 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PRAME transfected lysate.
Western Blot
Lane 1: PRAME transfected lysate ( 57.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PRAME full-length
Host
Human
Theoretical MW (kDa)
57.9
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PRAME antibody (H00023532-B01P) by Western Blots.
SDS-PAGE Gel
PRAME transfected lysate.
Western Blot
Lane 1: PRAME transfected lysate ( 57.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PRAME
Entrez GeneID
23532GeneBank Accession#
NM_006115.3Protein Accession#
NP_006106.1Gene Name
PRAME
Gene Alias
MAPE, OIP4
Gene Description
preferentially expressed antigen in melanoma
Omim ID
606021Gene Ontology
HyperlinkGene Summary
This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq
Other Designations
Opa-interacting protein OIP4|melanoma antigen preferentially expressed in tumors
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