PRAME MaxPab rabbit polyclonal antibody (D01)
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PRAME expression in transfected 293T cell line (H00023532-T02) by PRAME MaxPab polyclonal antibody.
Lane 1: PRAME transfected lysate(57.90 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PRAME transfected lysate using anti-PRAME MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRAME purified MaxPab mouse polyclonal antibody (B01P) (H00023532-B01P).
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to PRAME on HeLa cell. [antibody concentration 20 ug/ml]
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Specification
Product Description
Rabbit polyclonal antibody raised against a full-length human PRAME protein.
Immunogen
PRAME (NP_006106.1, 1 a.a. ~ 509 a.a) full-length human protein.
Sequence
MERRRLWGSIQSRYISMSVWTSPRRLVELAGQSLLKDEALAIAALELLPRELFPPLFMAAFDGRHSQTLKAMVQAWPFTCLPLGVLMKGQHLHLETFKAVLDGLDVLLAQEVRPRRWKLQVLDLRKNSHQDFWTVWSGNRASLYSFPEPEAAQPMTKKRKVDGLSTEAEQPFIPVEVLVDLFLKEGACDELFSYLIEKVKRKKNVLRLCCKKLKIFAMPMQDIKMILKMVQLDSIEDLEVTCTWKLPTLAKFSPYLGQMINLRRLLLSHIHASSYISPEKEEQYIAQFTSQFLSLQCLQALYVDSLFFLRGRLDQLLRHVMNPLETLSITNCRLSEGDVMHLSQSPSVSQLSVLSLSGVMLTDVSPEPLQALLERASATLQDLVFDECGITDDQLLALLPSLSHCSQLTTLSFYGNSISISALQSLLQHLIGLSNLTHVLYPVPLESYEDIHGTLHLERLAYLHARLRELLCELGRPSMVWLSANPCPHCGDRTFYDPEPILCPCFMPN
Host
Rabbit
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PRAME expression in transfected 293T cell line (H00023532-T02) by PRAME MaxPab polyclonal antibody.
Lane 1: PRAME transfected lysate(57.90 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of PRAME transfected lysate using anti-PRAME MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRAME purified MaxPab mouse polyclonal antibody (B01P) (H00023532-B01P).Immunofluorescence
Immunofluorescence of purified MaxPab antibody to PRAME on HeLa cell. [antibody concentration 20 ug/ml] -
Gene Info — PRAME
Entrez GeneID
23532GeneBank Accession#
NM_006115.3Protein Accession#
NP_006106.1Gene Name
PRAME
Gene Alias
MAPE, OIP4
Gene Description
preferentially expressed antigen in melanoma
Omim ID
606021Gene Ontology
HyperlinkGene Summary
This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq
Other Designations
Opa-interacting protein OIP4|melanoma antigen preferentially expressed in tumors
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Interactome
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